Genetic Engineering White Papers
Concerns about Gene Transfer and Nuclear Transfer in Domestic Animals
Overview Cell-cycle synchrony was not necessary in the older cloning methods because an aged enucleated oocyte was used. This oocyte does not allow nuclear envelope breakdown or a new zygotic one-cell DNA replication S phase of the cell cycle. Rather, the oocyte cleaves to two cell with mitotic division of the introduced nucleus. Enucleated early nonaged oocytes are essential to allow maximum genomic and cellular reprogramming of differentiated nuclei. An S phase and DNA replication will occur. Mistakes in incomplete DNA replication at the end of meiosis will either end in failed later embryo development or in interphase check point screening as the new cell enter mitosis. In mice, the cell fusion studies of Fulka et al. show that the mouse oocyte during meiosis has no cell cycle checkpoints, but screening for normal DNA replication does occur at interphase and later at M phase of the mitotic cell cycle.
| Publisher | National Institute of Standards and Technology | File Format | HTML |
|---|---|---|---|
| Date Published | August 2003 | Downloads | 11 |
| Format | White Papers | ||
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